Description
Overview
The Dual Wire Myograph System allows you to examine vascular reactivity and functional responses on small isolated arteries.
Features
Model 420A is ideal for testing two vessels with diameters of 30 μm simultaneously - 3 mm, independently. The vessels are mounted as ring preparations by threading them over two parallel wires and securing the wires to two supports or “jaws.” One support is attached to a precision micrometer, allowing manual control of vessel circumference and stretch. The other support is attached to a force transducer for the measurement of force/tension development.
The base of the chamber contains glass windows allowing morphological observations or fluorescence measurements on an inverted microscope. Typically, the preparation is kept in the heated vessel chamber in a physiological salt solution at 37°C, bubbled with oxygen where the vessels remain viable for at least 12 hours
The preparation is mounted in a heated 10 ml acid-resistant stainless steel chamber, which can be covered with a lid with ports for rapid suction/draining, refilling, and bubbling of oxygen supply. Following mounting and equilibration, the passive length-tension relationship of the vessel is determined. During the actual experiment, the circumference of the vessel is kept constant. Compounds can be added directly to the chamber, and the vessel’s contractility and reactivity are measured under isometric conditions.
In the Dual Wire Myograph System - 420A, the chamber can be divided for independent testing of each vessel. The two vessels also can be mounted in a single chamber by removing the divider. This allows direct comparative studies of vessels from treated/untreated conditions or diseased vs. healthy patient samples, for example, by exposing the vessels to identical concentrations of drug or compound. It is thus possible to examine whether a given pathological state is associated with altered morphology or reactivity.
As an option, an electronic valve can be added to the system for easy control and emptying of the chamber. The Wire Interface with a touch screen makes it easy to set up and use.
The Wire Interface with a touchscreen makes it easy to set up and use. MyoDAQ acquisition software is used to collect data. The data analysis is unique in being able to add multiple data selections throughout the course of the experiment in single or numerous channels. Data can be exported via a selection of the trace or the entire data file for external charting or custom analysis.
Feature
Ideal For Functional Studies Of Small Arteries
High Resolution And Precise Force Transducers
Direct Data Streaming to MyoDAQ
Modern & Today's Technology
Journal Citations
Nagaraja Nagre, et al., “Deletion of STIM1 in Treg cells protects against lung fibrosis and associated cardiovascular complications in a pre-clinical mouse model”, Redox Biology 92 (2026)
Miroslava Kvandova, et al., “Sex-Specific Cardiovascular Protection in Developing Metabolic Syndrome: The Role of AMPK”, Antioxidants2025
Anastasia A. Shvetsova, et al., “TASK-1 channel blockade by AVE1231 increases vasocontractile responses and BP in 1- to 2-week-old but not adult rats”, British Journal of Pharmacology, 2020
Rodriguez-Rodriguez, et al., “Oleanolic acid induces relaxation and calcium-independent release of endothelium-derived nitric oxide”, British Journal of Pharmacology, 2008
Morten Engholm, et al., “Involvement of transglutaminase 2 and voltage-gated potassium channels in cystamine vasodilatation in rat mesenteric small arteries”, British Journal of Pharmacology, 2008
